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KMID : 0896020080380030125
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Korean Journal of Oral and Maxillofacial Radiology
2008 Volume.38 No. 3 p.125 ~ p.132
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Effects of irradiation on TGF-β1 mRNA expression and calcific nodule formation
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Song Ju-Seop
Kim Kyoung-A
Koh Kwang-Joon
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Abstract
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Purpose : To investigate the effects of irradiation on transforming growth factor β1 (TGF-β1) mRNA expression and calcific nodule formation in MC3T3-E1 osteoblastic cell line.
Materials and Methods : Cells were cultured in alpha-minimum essential medium (α-MEM) supplemented with 10% fetal bovine serum and antibiotics. When the cells reached the level of 70-80% confluence, culture media were changed with α-MEM supplemented with 10% FBS, 5 mM β-glycerol phosphate, and 50 μg/mL ascorbic acid. Thereafter the cells were irradiated with a single dose of 2, 4, 6, 8 Gy at a dose rate of 1.5 Gy/min. The expression pattern of TGF-β1 mRNA, calcium content and calcific nodule formation were examined on day 3, 7, 14, 21, 28, respectively, after the irradiation.
Results : The amount of TGF-β1 mRNA expression decreased significantly on day 7 after irradiation of 4, 6, 8 Gy. It also decreased on day 14 after irradiation of 6, 8 Gy, and decreased on day 21 after irradiation of 8 Gy. The amount of calcium deposition decreased significantly on day 7 after irradiation of 4, 8 Gy (P?0.01) and showed a decreased tendency on day 14, 21 after irradiation of 4, 6, 8 Gy. The number of calcific nodules was decreased on day 7 after irradiation of 4, 8 Gy.
Conclusion : Irradiation with a single dose of 4, 6, 8 Gy influences negatively the bone formation at the molecular level by affecting the TGF-β1 mRNA expression that was associated with proliferation and the production of extracellular matrix in MC3T3-E1 osteoblastic cell line.(Korean J Oral Maxillofac Radiol 2008; 38 : 125-32)
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KEYWORD
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TGF-β1, MC3T3-E1 osteoblastic cell line, Irradiation
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